Type III ANOVA on main and interaction effects, and LM model summaries.
Prolactin
Chisq
Df
Pr(>Chisq)
(Intercept)
166.0537558
1
<0.0001
***
Folate
2.4526126
1
0.1
ns
GDM_new
0.6482207
1
0.4
ns
Study
1.3383219
1
0.2
ns
GA_samp
151.3636207
1
<0.0001
***
Folate:GDM_new
0.0737512
1
0.8
ns
GDM_new:Study
0.8231549
1
0.4
ns
Folate:Study
2.4443463
1
0.1
ns
Folate:GDM_new:Study
0.0167006
1
0.9
ns
HPL
Chisq
Df
Pr(>Chisq)
(Intercept)
37.7924677
1
<0.0001
***
Folate
0.1029730
1
0.7
ns
GDM_new
0.1600993
1
0.7
ns
Study
8.2930926
1
0.004
**
GA_samp
1945.3062005
1
<0.0001
***
Folate:GDM_new
0.2718582
1
0.6
ns
GDM_new:Study
0.1153311
1
0.7
ns
Folate:Study
0.0018958
1
1.0
ns
Folate:GDM_new:Study
0.9457358
1
0.3
ns
GH2
Chisq
Df
Pr(>Chisq)
(Intercept)
333.7985238
1
<0.0001
***
Folate
0.6363644
1
0.4
ns
GDM_new
0.0003137
1
1.0
ns
Study
0.2830153
1
0.6
ns
GA_samp
873.7248805
1
<0.0001
***
Folate:GDM_new
1.4214832
1
0.2
ns
GDM_new:Study
0.0000007
1
1.0
ns
Folate:Study
1.4248198
1
0.2
ns
Folate:GDM_new:Study
1.7380914
1
0.2
ns
Insulin
model term
df1
df2
F.ratio
Chisq
p.value
1
Folate
1
Inf
9.074
9.074
0.003
**
5
GDM_new
1
Inf
25.522
25.522
<0.0001
***
7
Study
1
Inf
9.643
9.643
0.002
**
8
GA_samp
1
Inf
0.032
0.032
0.9
ns
2
Folate:GDM_new
1
Inf
0.196
0.196
0.7
ns
4
Folate:Study
1
Inf
0.772
0.772
0.4
ns
6
GDM_new:Study
1
Inf
0.977
0.977
0.3
ns
3
Folate:GDM_new:Study
1
Inf
0.006
0.006
0.9
ns
HOMA.IR
model term
df1
df2
F.ratio
Chisq
p.value
1
Folate
1
Inf
11.114
11.114
0.0009
***
5
GDM_new
1
Inf
26.938
26.938
<0.0001
***
7
Study
1
Inf
15.817
15.817
<0.0001
***
8
GA_samp
1
Inf
0.005
0.005
0.9
ns
2
Folate:GDM_new
1
Inf
0.680
0.680
0.4
ns
4
Folate:Study
1
Inf
0.954
0.954
0.3
ns
6
GDM_new:Study
1
Inf
1.300
1.300
0.3
ns
3
Folate:GDM_new:Study
1
Inf
0.001
0.001
1.0
ns
GLUC3_mmol.L
model term
df1
df2
F.ratio
Chisq
p.value
1
Folate
1
Inf
3.341
3.341
0.07
ns
5
GDM_new
1
Inf
5.928
5.928
0.01
*
7
Study
1
Inf
17.784
17.784
<0.0001
***
8
GA_samp
1
Inf
0.589
0.589
0.4
ns
2
Folate:GDM_new
1
Inf
3.209
3.209
0.07
ns
4
Folate:Study
1
Inf
1.075
1.075
0.3
ns
6
GDM_new:Study
1
Inf
1.115
1.115
0.3
ns
3
Folate:GDM_new:Study
1
Inf
0.363
0.363
0.5
ns
59.2.2 Model summary
Ratio of Geometric means (95% CI)
Prolactin
Var
Effect
P
(Intercept)
2.68 (2.27, 3.09)
<0.0001
Folate
-0.00 (-0.00, 0.00)
0.11
GDM_newGDM
-0.14 (-0.38, 0.10)
0.25
StudySTOP
-0.10 (-0.26, 0.07)
0.25
GA_samp
0.15 (0.13, 0.17)
<0.0001
Folate:GDM_newGDM
0.00 (-0.00, 0.00)
0.68
GDM_newGDM:StudySTOP
0.17 (-0.06, 0.40)
0.14
Folate:StudySTOP
0.00 (-0.00, 0.00)
0.11
HPL
Var
Effect
P
(Intercept)
0.67 (0.46, 0.88)
<0.0001
Folate
-0.00 (-0.00, 0.00)
0.55
GDM_newGDM
-0.11 (-0.24, 0.01)
0.07
StudySTOP
0.12 (0.03, 0.21)
0.006
GA_samp
0.29 (0.28, 0.30)
<0.0001
Folate:GDM_newGDM
0.00 (-0.00, 0.00)
0.36
GDM_newGDM:StudySTOP
0.05 (-0.06, 0.17)
0.38
Folate:StudySTOP
0.00 (-0.00, 0.00)
0.70
GH2
Var
Effect
P
(Intercept)
-2.60 (-2.88, -2.32)
<0.0001
Folate
0.00 (-0.00, 0.00)
0.63
GDM_newGDM
-0.15 (-0.33, 0.02)
0.09
StudySTOP
0.02 (-0.10, 0.13)
0.79
GA_samp
0.26 (0.24, 0.28)
<0.0001
Folate:GDM_newGDM
0.00 (-0.00, 0.00)
0.93
GDM_newGDM:StudySTOP
0.18 (0.01, 0.34)
0.04
Folate:StudySTOP
-0.00 (-0.00, 0.00)
0.41
Insulin
Var
Effect
P
(Intercept)
2.73 (2.19, 3.27)
<0.0001
Folate
-0.00 (-0.00, -0.00)
0.01
GDM_newGDM
0.88 (0.51, 1.25)
<0.0001
StudySTOP
-0.36 (-0.58, -0.14)
0.001
GA_samp
0.00 (-0.03, 0.04)
0.86
Folate:GDM_newGDM
-0.00 (-0.00, 0.00)
0.62
GDM_newGDM:StudySTOP
-0.30 (-0.64, 0.04)
0.09
Folate:StudySTOP
0.00 (-0.00, 0.00)
0.25
HOMA.IR
Var
Effect
P
(Intercept)
1.38 (0.82, 1.95)
<0.0001
Folate
-0.00 (-0.01, -0.00)
0.010
GDM_newGDM
1.01 (0.60, 1.42)
<0.0001
StudySTOP
-0.52 (-0.75, -0.29)
<0.0001
GA_samp
-0.00 (-0.04, 0.03)
0.95
Folate:GDM_newGDM
-0.00 (-0.00, 0.00)
0.40
GDM_newGDM:StudySTOP
-0.36 (-0.73, 0.02)
0.06
Folate:StudySTOP
0.00 (-0.00, 0.00)
0.23
GLUC3_mmol.L
Var
Effect
P
(Intercept)
1.74 (1.63, 1.85)
<0.0001
Folate
-0.00 (-0.00, 0.00)
0.24
GDM_newGDM
0.17 (0.03, 0.31)
0.02
StudySTOP
-0.16 (-0.21, -0.12)
<0.0001
GA_samp
-0.00 (-0.01, 0.00)
0.46
Folate:GDM_newGDM
-0.00 (-0.00, -0.00)
0.02
GDM_newGDM:StudySTOP
-0.08 (-0.19, 0.04)
0.19
Folate:StudySTOP
0.00 (-0.00, 0.00)
0.18
59.2.3 Estimated means
Estimated marginal means (or adjusted means) adjusted for GA at sampling, BMI, ethnicity and smoking.
Results are averaged over the levels of Serum folate concentration (nM) and may be misleading when interaction term is involved.
Prolactin
Study
GDM_new
response
SE
df
lower.CL
upper.CL
SCOPE
Uncomplicated
91.93238
7.620145
113.41538
78.01044
108.3389
STOP
Uncomplicated
93.81986
7.063797
77.74118
80.75951
108.9923
SCOPE
GDM
82.63955
10.578321
491.25986
64.26290
106.2712
STOP
GDM
100.24496
8.918948
146.46724
84.08120
119.5160
HPL
Study
GDM_new
response
SE
df
lower.CL
upper.CL
SCOPE
Uncomplicated
85.75586
2.653914
225.3123
80.68246
91.14828
STOP
Uncomplicated
98.24072
2.536388
111.6939
93.34143
103.39717
SCOPE
GDM
79.67707
4.566763
659.9960
71.19612
89.16828
STOP
GDM
96.21510
3.504870
338.2123
89.56221
103.36219
GH2
Study
GDM_new
response
SE
df
lower.CL
upper.CL
SCOPE
Uncomplicated
2.260977
0.0815629
556.9267
2.106312
2.426998
STOP
Uncomplicated
2.198475
0.0589499
224.5472
2.085325
2.317765
SCOPE
GDM
1.949570
0.1550901
1035.3101
1.667807
2.278935
STOP
GDM
2.261217
0.0965979
534.4997
2.079203
2.459165
Insulin
Study
GDM_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
Uncomplicated
13.38897
0.8605354
Inf
11.804264
15.18643
STOP
Uncomplicated
10.25874
0.4219833
Inf
9.464133
11.12007
SCOPE
GDM
30.82520
4.5651816
Inf
23.059175
41.20671
STOP
GDM
17.52716
1.3652257
Inf
15.045605
20.41800
HOMA.IR
Study
GDM_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
Uncomplicated
3.234789
0.2205633
Inf
2.830133
3.697302
STOP
Uncomplicated
2.135520
0.0926688
Inf
1.961402
2.325095
SCOPE
GDM
8.116638
1.3208034
Inf
5.900135
11.165815
STOP
GDM
3.741720
0.3114622
Inf
3.178462
4.404795
GLUC3_mmol.L
Study
GDM_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
Uncomplicated
5.408408
0.0700182
Inf
5.272901
5.547397
STOP
Uncomplicated
4.722556
0.0455694
Inf
4.634081
4.812720
SCOPE
GDM
5.947076
0.2916597
Inf
5.402048
6.547094
STOP
GDM
4.814860
0.0609634
Inf
4.696845
4.935841
59.2.4 Comparisons
59.2.4.1 Within Groups
Effect for every 50 unit increase in Serum folate concentration (nM).
Hormone
Contrast
Study
GDM_new
Ratio of Geometric means (95% CI)
P-value
Prolactin
Folate89.2 / Folate39.2
SCOPE
Uncomplicated
0.928 (0.846, 1.018)
0.1
Prolactin
Folate89.2 / Folate39.2
STOP
Uncomplicated
1.017 (0.950, 1.088)
0.6
Prolactin
Folate89.2 / Folate39.2
SCOPE
GDM
0.953 (0.822, 1.105)
0.5
Prolactin
Folate89.2 / Folate39.2
STOP
GDM
1.044 (0.935, 1.165)
0.4
HPL
Folate89.2 / Folate39.2
SCOPE
Uncomplicated
0.986 (0.940, 1.034)
0.5
HPL
Folate89.2 / Folate39.2
STOP
Uncomplicated
0.997 (0.961, 1.034)
0.9
HPL
Folate89.2 / Folate39.2
SCOPE
GDM
1.018 (0.941, 1.101)
0.7
HPL
Folate89.2 / Folate39.2
STOP
GDM
1.030 (0.969, 1.094)
0.3
GH2
Folate89.2 / Folate39.2
SCOPE
Uncomplicated
1.016 (0.951, 1.086)
0.6
GH2
Folate89.2 / Folate39.2
STOP
Uncomplicated
0.982 (0.935, 1.032)
0.5
GH2
Folate89.2 / Folate39.2
SCOPE
GDM
1.021 (0.916, 1.138)
0.7
GH2
Folate89.2 / Folate39.2
STOP
GDM
0.987 (0.910, 1.071)
0.7
Insulin
Folate89.2 / Folate39.2
SCOPE
Uncomplicated
0.872 (0.784, 0.971)
0.01
Insulin
Folate89.2 / Folate39.2
STOP
Uncomplicated
0.938 (0.870, 1.011)
0.09
Insulin
Folate89.2 / Folate39.2
SCOPE
GDM
0.840 (0.713, 0.989)
0.04
Insulin
Folate89.2 / Folate39.2
STOP
GDM
0.903 (0.782, 1.043)
0.2
HOMA.IR
Folate89.2 / Folate39.2
SCOPE
Uncomplicated
0.862 (0.770, 0.965)
0.010
HOMA.IR
Folate89.2 / Folate39.2
STOP
Uncomplicated
0.935 (0.863, 1.013)
0.10
HOMA.IR
Folate89.2 / Folate39.2
SCOPE
GDM
0.804 (0.676, 0.957)
0.01
HOMA.IR
Folate89.2 / Folate39.2
STOP
GDM
0.873 (0.751, 1.014)
0.08
GLUC3_mmol.L
Folate89.2 / Folate39.2
SCOPE
Uncomplicated
0.988 (0.969, 1.008)
0.2
GLUC3_mmol.L
Folate89.2 / Folate39.2
STOP
Uncomplicated
1.011 (0.988, 1.034)
0.4
GLUC3_mmol.L
Folate89.2 / Folate39.2
SCOPE
GDM
0.935 (0.881, 0.992)
0.03
GLUC3_mmol.L
Folate89.2 / Folate39.2
STOP
GDM
0.956 (0.922, 0.992)
0.02
59.2.4.2 Between Groups
Below are comparisons of the differences in the change in Serum folate concentration (nM) between groups. I.e. whether the change in Serum folate concentration (nM) are different between groups.